Biotehnoloogia magistritööd - Master's theses

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Browsing Biotehnoloogia magistritööd - Master's theses by Author "Bottoms, Scott, juhendaja"

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    Deploying Pseudomonas putida for the conversion of aromatic compounds from fractionated industrial hydrolysis lignin
    (Tartu Ülikool, 2023) Morehead, Philip Arthur; Bottoms, Scott, juhendaja; Salmar, Siim, juhendaja; Loog, Mart, juhendaja; Tartu Ülikool. Loodus- ja täppisteaduste valdkond; Tartu Ülikool. Tehnoloogiainstituut
    The world’s most abundant natural aromatic polymer is paradoxically one of its most un- derutilized feedstocks. Lignin is the pulp and paper industry’s primary by-product, and it shows great potential as a renewable and carbon-neutral source of industrially relevant chemicals. The utilization of Pseudomonas putida, a robust bacterium known to catabolize aromatic compounds natively, was explored in this study as a promising approach for lignin valorization. To this end, dry hydrolysis lignin (HL) was used as the feedstock, and various HL fractionation techniques were applied, including alkaline extraction, to obtain lignin monomers, dimers, and trimers in solution for use as a growth medium. The composition of the fractionated HL media was ascertained using a range of analytical techniques. Changes in composition during flask cultivations helped inform the selection of target genes for deletion to direct compounds in the benzoate degradation pathway towards the production of cis,cis-muconic acid, an intermediate of nylon 6,6, which finds applications in industrial components, textiles, automotive parts, and electronics.
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    Development Of Glucose-independent Yarrowia Lipolytica Processes
    (Tartu Ülikool, 2024) Kucukozden, Yigit; Bottoms, Scott, juhendaja; Dahlin, Jonathan, juhendaja; Mans, Robert, juhendaja; Tartu Ülikool. Loodus- ja täppisteaduste valdkond; Tartu Ülikool. Tehnoloogiainstituut
    As the global population continues to expand, there is a rising demand for animal meat production. However, the consumption and production of animal-based protein have detrimental effects on human health and the environment. Furthermore, challenges such as zoonotic diseases, climate change, and land scarcity underscore the need for exploring alternative meat sources. The study aims to investigate the feasibility of using Yarrowia lipolytica in mycoprotein research, focusing on enhancing its growth rate on environmentally friendly carbon sources that mitigate industrial CO2 emissions. This involves employing molecular and genetic approaches to optimize natural metabolic pathways and utilizing adaptive laboratory methods for fermentation. This study, a part of the Yummowia project, advances scientific understanding in synthetic biology and food science and holds promise in shaping the future landscape of sustainable and appealing meat alternatives. The collaborative and multidisciplinary nature sets the stage for continued innovation, with the potential to positively influence the broader food industry.