Browsing by Author "Reinsalu, Olavi, supervisor"

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    Flow cytometry analysis of fluorescently-labelled extracellular vesicles
    (Tartu Ülikool, 2019) Rajabli, Naila; Reinsalu, Olavi, supervisor; Kurg, Reet, supervisor
    In English: In the recent decade, there was a rise of interest in extracellular vesicles (EVs) due to their potential as biomarkers and drug delivery. However, for better understanding of extracellular vesicles standardized isolation and detection protocols that would take into account their heterogeneity and small size are needed. The present thesis studies the efficiency of studying fluorescently tagged extracellular vesicles by flow cytometry and the challenges of this method. Taken together, the findings in this thesis suggest that flow cytometry has the potential to be utilized as an EVs analysis method but after all, further studies are needed for enhancing small particles detection and reading noise decreasing. Eesti keeles:Viimase kümnendi jooksul on ekstratsellulaarsete vesiikulite (EV), kui potentsiaalselt heade biomarkerite ja ravimikandjate, vastu tõusnud suur teaduslik huvi. Kuigi standartsed EV-de puhastamise ning tuvastamise meetodid, mis võimaldaksid EV-sid paremini tundmaõppida, vajavad veel väljatöötamist. Käesolevas uurimustöös hinnatakse voolutsütomeetria sobivust fluorestsentselt märgistatud EV-de analüüsimiseks ning tuuakse välja selle puudused. Kokkuvõtvalt, uurimustöö tulemuste põhjal võib väita, et voolutsütomeetria on potentsiaali olla sobiv meetod EV-de uurmiseks, kuid on vaja teostada edasisi uuringuid väikseste partiklite tuvastamise parandamiseks ning taustamüra vähendmiseks.