Construction and analysis of Far1 based synthetic inhibitor protein for Cln2-Cdk1

Abstract

The complex cell divides the process into distinct and more easily attainable events, allowing it to reproduce itself with exceptional precision. This highly regulated series of events is known as the cell cycle. The enzymes called cyclin-dependant kinases (Cdks) are the chief constituents of the cell cycle control system and are activated by binding to regulatory proteins called cyclins. Cell cycle progression requires accurately ordered inhibition of cyclins and Cdk inhibitor proteins (CKIs) by ubiquitination. Far1: an inhibitor of G1/S cyclin-Cdk complexes in response to mating pheromones can arrest the cell cycle in the G1 phase. In this study, a docking site from Sic1 (an inhibitor of S and M Cdk complexes) responsible for phosphorylation was put to N-Far1(1-170aa) to make a synthetic Cln2 specific inhibitor and test its ability to cause cell cycle arrest in the G1 phase.