Controlling protein levels through Grr1- dependent synthetic degrons

Abstract

The cell cycle control system, governed by cyclin-dependent kinases (Cdks), orchestrates the precise timing and coordination of cell cycle events. Ubiquitination-mediated protein degradation, facilitated by the Skp1-Cullin-F-box protein (SCF) complex and one of its F- box proteins, Grr1, is pivotal in regulating cell cycle progression. This study explores using phosphodegron tagging while incorporating various modules to control protein expression and degradation. The development of phosphodegron through synthetic biology has great potential for practical uses, including improving production yields for valuable compounds.